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RNA-seq analysis β-catenin-silenced or control 293FT cells were treated with CPT (10 μM, 4 h), and the total RNA was extracted and purified using the Trizol (Life Technologies) reagent according to the manufacturer’s instruction

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Digital sensor readouts, cognitive composites for GBA1 carriers, CSF α-syn seeds, and PET-derived synaptic density all show promise, but each is validated in isolation, on limited numbers, and with divergent statistical cut-offs [306,307,308]